Abstract

This is the first phytochemical investigation of the roots of Calophyllum pisiferum Planch. & Triana. A new 4-phenyl coumarin, calopisifuran (1), and a new xanthone, 1-hydroxy-4,5-dimethoxyxanthone (2), were isolated and identified, together with 11 known compounds, including 4-phenyl coumarin (3-7) and xanthones (8-13). Their structures were elucidated by 1D- and 2D-NMR spectra and by HR-ESI-QTOF mass spectra and were compared with the literature data for known compounds. The isolated compounds exhibited significant cytotoxic activity in the MDA-MB-231, MCF-7 and A-549 cell lines, with IC<inf>50</inf> values of 14.87, 23.56 and 43.34 µg/mL, respectively, for 10 and IC<inf>50</inf> values of 17.15, 45.76 and 85.17 µg/mL, respectively, for 1, while the DPPH assay revealed weak antioxidant activity.