Abstract

Three new caryophyllene sesquiterpenes, pestalotiopsins I–K, one new chromone, pseudopestalone, and one new 10-membered macrolide, decarestrictine Q, were isolated from the fermentation broth of the marine-derived fungus Pseudopestalotiopsis sp. PSU-AMF45 along with twelve known compounds. Their structures were identified by spectroscopic data including 1D and 2D NMR. The absolute configuration of pestalotiopsin I was assigned based on single-crystal X-ray diffraction crystallography whereas that of the secondary alcohol moiety in pestalotiopsin J was established by Mosher's method. For pseudopestalone, the absolute configuration was determined by ECD spectrum associated with TD-DFT calculation. The antimicrobial activity of the isolated compounds was evaluated.