Journal of Pediatric Infectious Diseases, Volume 12, Issue 4, Pages 249-255 , 01/12/2017
Malaria in Children: Diagnostic Tools in Resource-Limited Settings
Abstract
Malaria contributes to a major reason for the cause of fatality and serious health problems among children mainly in various parts of tropical Africa and Asia. The extraction of DNA plays a vital role in the diagnosis of this life-threatening parasite Plasmodium. Difficulties with existing extraction methods of DNA are increasing with time including detection limits, time duration, sensitivity, availability of chemicals, and cost. Consequently, there is now renewed interest in finding of novel methods for DNA extraction of Plasmodium. However, the extraction of DNA by using microwave irradiation is found to be most convenient and suitable due to its low-cost and usage of common chemical reagents especially for the children in poor resource settings. In addition, the low cost, noninvasive malaria diagnostics tests are needed to carry out particularly for children to avoid accidental infections which may happen due to drawing blood.
Document Type
Review
Source Type
Journal
Keywords
DNA extractionmalarianoninvasiveplasmodium
ASJC Subject Area
Medicine : Pediatrics, Perinatology and Child HealthMedicine : Infectious Diseases