Abstract

Protein isolates from broiler meat were produced using acid-aided process (AP) with some modifications including prewashing prior to pH shift process (PW), homogenisation with 5 mM citric acid plus 8 mM CaCl<inf>2</inf> (CA) and homogenisation with 5 % ethanol (EA). Significant reduction of total cholesterol, nucleic acids and haemoproteins with improved protein yield and whiteness of the isolate was obtained from all modified process (p < 0.05). CA was the most effective method in elimination of cholesterol (p < 0.05). However, PW was the best way to remove nucleic acids and haem proteins (p < 0.05). Among modified processes, CA gel tended to show the highest breaking force, deformation and whiteness but PW gel had the lowest expressible drip (p < 0.05). EA possessed a negative effect on gel-forming ability. Therefore, CA or PW was a promising means to produce functional protein isolate with reduced nucleic acids, haem pigments and cholesterol.