Abstract

To investigate the prevalence of H. pylori infection in dyspepsia patients and it relation to virulence factor cagA gene. In total, 110 gastric biopsies from dyspeptic patients were comparatively studied using rapid urease test and multiplex PCR. Multiplex PCR detected three genes of 16S rBNA, cagA, and ureC. H. pylori was detected in 14 gastric biopsies (13%). Significantly higher numbers of female were infected. Furthermore, cagA gene was found in all H. priori-positive specimens. In addition, the result indicated that the multiplex PCR with annealing temperature at 57 °C was able to effectively amplify specific products. The results confirmed that high preva-lence of cagA gene in H. pylori among dyspeptic patients in Southern Thailand.