Abstract

Modifying the titanium alloy (Ti-6Al-4V) surface plays a key role in improving biological responses. The present work aimed to determine the response of human alveolar bone cells to the diamond-like carbon (DLC) film and to evaluate the antibacterial effect of DLC-coated titanium. The alloys were deposited with different compositions, including C<inf>2</inf>H<inf>2</inf> (DLC), carbon tetrafluoride (CF<inf>4</inf>:C<inf>2</inf>H<inf>2</inf>; F-DLC), and tetramethylsilane (Si(CH<inf>3</inf>)<inf>4</inf>:C<inf>2</inf>H<inf>2</inf>; Si-DLC). The surface morphology and wettability were measured using an atomic force microscopy (AFM) and a contact angle measurement. Titanium coated with DLC, F-DLC, or Si-DLC increased surface roughness and hydrophobicity. The ratio of spreading cells to initial rounded attaching cells at 20 min of all modified surfaces was higher than that of the control surfaces. F-DLC showed better cell spreading at 20 min compared to other conditions, as observed by scanning electron microscopy (SEM). The DLC, F-DLC, and Si-DLC coating significantly promoted cell proliferation compared to the Ti-6Al-4V control surfaces. ALP mRNA expression was significantly reduced on DLC surfaces compared to the control on day 14. Additionally, ALP and BMP2 mRNA expression increased on F-DLC-coated surfaces, but there were no statistically significant differences. However, DLC surfaces promoted higher mineral deposition than the control. The lower Staphylococcus aureus colony was observed on the surface of the DLC and F-DLC. In conclusion, the DLC-modified Ti-6Al-4V surface modulates cell attachment, proliferation, osteogenic marker gene expression, and bacterial growth. Herein, DLC-coated titanium alloys are still needed for continued development as an alternative biomaterial in dental applications.