Abstract

Compared with the use of a single herb alone, herb combinations can signifcantly increase their efectiveness in treating various diseases. The objective of this research was to determine the appropriate proportions of Garcinia mangostana (GM), Curcuma comosa (CC), and Acanthus ebracteatus (AE) to enhance their antioxidant and anti-infammatory properties. The study employed computer modeling (in silico) and laboratory testing (in vitro). The optimal levels of phenolic and favonoid compounds were achieved with a ratio of 15 parts GM, 5 parts CC, and 10 parts AE. In contrast, the 10: 15: 5 ratio resulted in the highest antioxidant activity, reducing DPPH radicals by 80.33% and ABTS radicals by 92.20%. The ratio had a synergistic efect and was within the safe range, ranging from 82.97 to 147.04 µg/mL. The ratio of 5: 10: 15 had the greatest anti-infammatory efects, with an exceptional activity level of 98.86%. It efectively suppressed 23 genes or proteins, including the prominent NFE2L2 gene, and had a synergistic efect. This combination efectively inhibits infammation (IC<inf>50</inf>: 12.05 µg/mL) and is safe for macrophages at concentrations up to 50 µg/mL. These fndings emphasize the potential of these herbal mixtures for antioxidant and anti-infammatory applications.